DIY CRISPR Kit

DIY CRISPR Kit

Product Description

There is currently ~1 week time till shipment.

Due to the overwhelming number of emails we will not respond to emails asking when your item will be shipped. Understand we are doing our best to get it to you.

Comes with an example experiment that teaches you many molecular biology and gene engineering techniques.

Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering? This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, tracrRNA, crRNA and Template DNA template for an example experiment.

Includes example experiment to make a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) thereby allowing the bacteria to survive on Strep media which would normal prevent its growth.

Kit contains enough materials for around 5 experiments or more

  • LB Agar
  • LB Strep/Kan/Arabinose Agar
  • Glass bottle for pouring plates
  • Non-pathogenic E. coli bacteria
  • Inoculation Loops/Plate Spreader
  • 10-100uL variable volume adjustable pipette(1uL increments)
  • Box of 96 Pipette Tips
  • 14 Petri Plates
  • Microcentrifuge tube rack
  • Nitrile Gloves
  • Microcentrifuge tubes
  • 50mL Tube for measuring
  • Bacterial transformation buffer 25mM CalCl2, 10% PEG 8000
  • LB Media for transformation recovery
  • Cas9 plasmid
  • gRNA plasmid
  • Template DNA

Source: DIY CRISPR Kit

Harvard scientists to make LSD factory from microbes 

Skip to main content current edition: US edition The Guardian – Back to home Make a contribution Subscribe Find a job Sign in Search News Opinion Sport Culture Lifestyle Show More US World Environment World Cup 2018 Soccer US politics Business Tech Science Homelessness Science Notes & Theories Harvard scientists to make LSD factory from microbes Simple microbes such as those found in baker’s yeast can be modified to make LSD, suggests research by Harvard scientists Ian Sample @iansample Tue 21 Jun 2011

Source: Harvard scientists to make LSD factory from microbes | Science | The Guardian